Disease is now considered to be the limiting factor in aquaculture. Often it caused great financial loss. Disease can cause mortality, cretinism, longly maintain period, highly feed convertion, lower density and decrease of productivity. Disease can be caused by pathogen organism such as protozoa, bacteria, fungi and virus.
Vibriosis is one of bacterial disease caused by vibrionaceae family, the effect of the attack is massive. So far, disinfectants and antimicrobial drugs are used to treat this disease. The continous use of antibiotic to treat this disease is causing resistancy at pathogen bacteria. They can transfer their resistance genes to other bacteria that have never been exposed to the antibiotic. Probiotic is a necessary developed alternative to treat vibriosis. The result of this tecnology is a safe product to consumer because it does not contain chemical residue that is dangerous to our health.
The aim of the research was to explorate bacteria associated with soft coral Lobophyton sp. as probiotic candidate to control vibriosis that caused by pathogenic bacteria.
This research was done at June-September 2004 in Microbiology Laboratory and Processing Laboratory of Marine Science Department, Teluk Awur, Jepara, whereas the identification of bacteria was held in Pest and Disease of Fish and Shrimp Laboratory BBPBAP Jepara. The sampling of the soft coral Lobophyton sp. was taken in Panjang Island Waters Jepara. This research uses experimental laboratories methode. Data was analyzed by descriptive and Anova.
Based on this research, 15 bacterial isolates has been obtained and seven isolates (A, B, E, F, J, K and O) selected as probiotic candidates. Isolate bacteria with B code was the best candidate because it can inhibit three tester bacteria and it was identified as V. splendidus. Bacteria with J code (V. marinus) can increase survival rate of shrimp, and the other candidates decrease the survival rate, but Anova test showed no certain difference, so they can still be used as probiotic candidates. Bacteria with E and F code identified as V. pelagius II. Mix culture probiotic recomended to control vibriosis are between V. splendidus and K isolate bacteria or between V. splendidus, V. pelagius II, V. marinus and K isolate.
Keywords: bacteria isolate, disease, vibriosis, probiotic, Lobophyton sp.
Author: Muhammad Najib Habibie
Advisors: Subagiyo and Wilis Ari Setyati
Vibriosis is one of bacterial disease caused by vibrionaceae family, the effect of the attack is massive. So far, disinfectants and antimicrobial drugs are used to treat this disease. The continous use of antibiotic to treat this disease is causing resistancy at pathogen bacteria. They can transfer their resistance genes to other bacteria that have never been exposed to the antibiotic. Probiotic is a necessary developed alternative to treat vibriosis. The result of this tecnology is a safe product to consumer because it does not contain chemical residue that is dangerous to our health.
The aim of the research was to explorate bacteria associated with soft coral Lobophyton sp. as probiotic candidate to control vibriosis that caused by pathogenic bacteria.
This research was done at June-September 2004 in Microbiology Laboratory and Processing Laboratory of Marine Science Department, Teluk Awur, Jepara, whereas the identification of bacteria was held in Pest and Disease of Fish and Shrimp Laboratory BBPBAP Jepara. The sampling of the soft coral Lobophyton sp. was taken in Panjang Island Waters Jepara. This research uses experimental laboratories methode. Data was analyzed by descriptive and Anova.
Based on this research, 15 bacterial isolates has been obtained and seven isolates (A, B, E, F, J, K and O) selected as probiotic candidates. Isolate bacteria with B code was the best candidate because it can inhibit three tester bacteria and it was identified as V. splendidus. Bacteria with J code (V. marinus) can increase survival rate of shrimp, and the other candidates decrease the survival rate, but Anova test showed no certain difference, so they can still be used as probiotic candidates. Bacteria with E and F code identified as V. pelagius II. Mix culture probiotic recomended to control vibriosis are between V. splendidus and K isolate bacteria or between V. splendidus, V. pelagius II, V. marinus and K isolate.
Keywords: bacteria isolate, disease, vibriosis, probiotic, Lobophyton sp.
Author: Muhammad Najib Habibie
Advisors: Subagiyo and Wilis Ari Setyati
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